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ClariTrial

IRAK4

Serine/Threonine Kinase (Scaffolding Kinase)Kymera Only (TPD)

Interleukin-1 Receptor-Associated Kinase 4

Protein length
460 aa
ChEMBL activities
31,578
Disease areas
4
Kymera program
Phase 2

IRAK4 is the central scaffold kinase that assembles the myddosome signaling complex downstream of all Toll-like receptors (TLR1-9 except TLR3) and all IL-1 family receptors. It drives innate immune responses through NF-κB and MAPK pathways. Critically, IRAK4 has both kinase-dependent and kinase-independent (scaffolding) functions — making it a uniquely compelling degradation target where inhibiting kinase activity alone is insufficient.

Key Facts

  • Sanofi partnership: up to $2.1B total potential, $150M upfront (2020)
  • Only degrader approach to IRAK4 — eliminates scaffolding function that inhibitors miss
  • KT-474 Phase 1 validated human proof-of-concept in AD and HS patients
  • KT-485 is 2nd-generation with improved potency and selectivity
  • Sanofi leads Phase 2; Kymera retains US co-development option (50/50 split)
  • Addresses TLR/innate immune pathway across 8+ inflammatory indications

Mechanism of Action

TLR/IL-1R ligation triggers recruitment of MyD88 adaptor protein, which oligomerizes and recruits IRAK4 via death domain interactions. IRAK4 auto-phosphorylates and phosphorylates IRAK1/2, triggering TRAF6 recruitment, K63-ubiquitin chain formation, TAK1 activation, and ultimate NF-κB/AP-1/IRF5 nuclear translocation and pro-inflammatory cytokine production (TNFα, IL-6, IL-8, IL-1β, IL-12, IL-18). IRAK4 also scaffolds the myddosome complex independently of kinase activity — making complete protein degradation far more effective than kinase inhibition.

Signaling Pathway

1
TLR/IL-1R activation
PAMPs, DAMPs, or IL-1 family cytokines activate TLR or IL-1R family receptors at the cell surface or endosome (TLR7/9).
2
MyD88 oligomerization
MyD88 adaptor is recruited and oligomerizes via TIR domain interactions, forming a helical oligomeric platform for IRAK4 recruitment.
3
Myddosome assembly
IRAK4 is recruited to the MyD88 oligomer via death domain interactions, bringing in IRAK1/2 to complete the myddosome. This step requires IRAK4 protein presence regardless of kinase activity.
4
IRAK4 auto-phosphorylation and IRAK1/2 phosphorylation
IRAK4 kinase activity phosphorylates IRAK1 at multiple sites, triggering IRAK1 hyperphosphorylation and dissociation from the complex.
5
TRAF6 activation
Dissociated p-IRAK1 recruits TRAF6, triggering K63-polyubiquitination. Ubiquitin chains recruit TAK1/TAB complex.
6
NF-κB and MAPK activation
TAK1 activates IKK complex → IκBα phosphorylation and degradation → NF-κB nuclear translocation. Simultaneously, TAK1 activates MAPK pathway → AP-1 transcription.
7
Pro-inflammatory cytokine production
NF-κB and AP-1 drive TNFα, IL-6, IL-8, IL-1β, IL-12, IL-18 expression — the core cytokines driving HS, AD, RA, and other inflammatory diseases targeted by KT-485.

Why Degradation

IRAK4 kinase inhibitors (e.g., PF-06650833/Emfilerminib, BAY-1830839) showed efficacy in RA but were incomplete because IRAK4's scaffolding function persisted. The myddosome assembly still occurs when IRAK4 kinase is inhibited. Protein degradation eliminates both the kinase AND scaffolding functions simultaneously — a fundamental advantage over inhibitors. Kymera's predecessor KT-474 proved this concept in human Phase 1, demonstrating superior pathway suppression vs. kinase-only inhibition.

Previously Attempted Approaches

IRAK4 kinase inhibitors: PF-06650833/emfilerminib (Pfizer, Phase 2 in RA/IBD — showed partial efficacy), BAY-1830839 (Bayer, discontinued), M3258 (Merck KGaA, preclinical). None achieved full pathway blockade due to scaffolding function persistence.

Compound Potency (ChEMBL)

pChEMBL = −log₁₀(IC₅₀). Higher value = more potent. Dashed line = 100 nM threshold. Showing top binding compounds in ChEMBL (31,578 total activities).

View all 31,578 activities on ChEMBL

Kymera Program

Phase 2
KT-485 / SAR447971
Partner: Sanofi

2nd-generation IRAK4 degrader designed with superior potency and CNS selectivity vs. first-gen KT-474. Predecessor KT-474 completed Phase 1 in healthy volunteers and patients with HS and AD, providing proof-of-concept for IRAK4 degradation as a therapeutic mechanism in humans. Sanofi is sponsoring and leading Phase 2 development.

Clinical Data

KT-474 Phase 1 (2022): Proof-of-concept in atopic dermatitis patients. Deep IRAK4 degradation observed in PBMCs. Reductions in inflammatory cytokines (TNFα, IL-6, IL-8). Favorable safety profile.

Target Indications
Hidradenitis SuppurativaAtopic DermatitisRAAsthmaIBDPsoriasisGPPSLE
Full Kymera Profile

Competitive Context

No TPD Competitors

No other company has a targeted protein degrader program against IRAK4 in the clinic. Kymera holds first-mover advantage in this target.

View full TPD landscape

Clinical Endpoints

HiSCR75Hidradenitis Suppurativa Clinical Response 75%

≥75% reduction in total abscess and nodule count (AN) with no increase in draining tunnels or abscesses. Primary endpoint for HS trials.

IGA-HS 0/1IGA for Hidradenitis Suppurativa Clear/Almost Clear

Score of 0 (clear) or 1 (almost clear) with ≥2-point improvement from baseline. Key secondary in HS.

ACR20/50/70American College of Rheumatology Response Criteria

Standard RA primary endpoints. ACR50/70 are increasingly preferred in trials.

DAS28-CRPDisease Activity Score 28 joints (CRP)

Composite RA disease activity measure using 28 joint counts plus CRP.

Disease Context

Total patient potential: Broad immune-inflammatory populations: HS (~3M US), AD (~16M US), RA (~1.3M US), IBD (~3M US)

Hidradenitis Suppurativa (HS)
Prevalence
~1% of the US population (~3.3M); predominantly young adults
Market Leader
Humira (adalimumab) — only FDA-approved biologic for HS; Bimekizumab, Secukinumab
Unmet Need
Majority of patients don't achieve HiSCR75 on TNF inhibitors. High disease burden; HS is associated with severe quality-of-life impairment. An oral option would address major unmet need.
Atopic Dermatitis (AD)
Prevalence
230M globally; 16M US adults
Market Leader
Dupixent (dupilumab), Cibinqo (abrocitinib), Rinvoq (upadacitinib)
Unmet Need
Combination with STAT6 degrader KT-621 could provide complementary innate + adaptive immune suppression.
Rheumatoid Arthritis (RA)
Prevalence
1.3M in the US; 18M globally
Market Leader
Humira, Enbrel, Rinvoq, Olumiant (baricitinib)
Unmet Need
Patients cycling through multiple biologics and JAK inhibitors. IRAK4 degradation offers a distinct mechanism.
IBD (Crohn's / UC)
Prevalence
3M+ in the US combined
Market Leader
Stelara, Skyrizi, Entyvio
Unmet Need
Significant proportion of biologic non-responders. IRAK4/TLR pathway implicated in gut mucosal inflammation.
Search PubMed for IRAK4 literature
IRAK4 degrader PROTAC · KT-474 hidradenitis suppurativa
Open PubMed