This observational study aims to detect levels of iron, alpha-synuclein, and soluble lymphocyte activation gene 3 in acute ischemic stroke patients. And to see expressions of iron, alpha-synuclein, and lymphocyte activation gene 3 in the brain tissue of ischemic rats. The main questions it aims to answer are: * Is there an association between iron and alpha-synuclein accumulation in ischemic stroke? * Is there any change in soluble lymphocyte activation gene levels in ischemic stroke and if these levels are related to stroke severity and infarction size? . Can soluble lymphocyte activation gene levels be used as an early biomarker to diagnose ischemic stroke?
Inclusion Criteria: 1. Patients at the age between 50-70 years. 2. Patients having neurological symptoms of acute ischemic stroke. Exclusion Criteria: 1. Patients with acute hemorrhagic stroke. 2. Patients with Parkinson's disease (PD). 3. Patients with dementia, and Alzheimer's disease. 4. Patients with any type of malignancy. 5. Patients with central nervous system infection.