Breast lesions of uncertain malignant potential represent a diagnostic challenge, as conventional histopathological assessment does not always reliably distinguish between benign and malignant changes. The purpose of this prospective diagnostic study is to evaluate whether methylation patterns of selected breast cancer-related genes (BRCA1, RASSF1A, and PTEN) can help differentiate benign from malignant breast lesions. Tissue samples obtained during diagnostic needle biopsy, and when applicable during surgical excision, will be analyzed for gene methylation status. The results will be compared with standard histopathological findings. The study aims to improve diagnostic accuracy in breast lesions of uncertain malignant potential and contribute to better clinical decision-making in breast diagnostics.
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Inclusion Criteria: * Female patients aged 18 years or older. * Patients referred for diagnostic evaluation of breast lesions of uncertain malignant potential. * Availability of breast tissue samples obtained by diagnostic needle biopsy. * Written informed consent for participation in the study. Exclusion Criteria: * Male patients. * Patients younger than 18 years. * Patients without breast lesions or without indication for diagnostic biopsy. * Inadequate or insufficient tissue material for molecular analysis. * Withdrawal of informed consent.