The investigators will investigate the effects of acute and sub-acute administration of gluten on mood, intestinal permeability, gastrointestinal symptoms and gut peptide levels in healthy volunteers (HV).
Inclusion Criteria: * Body Mass Index (BMI) of 20 - 25 kg/m2 * Stable body weight for at least 3 months prior to the start of the study Exclusion Criteria: * Medical * Coeliac disease (excluded either by absence of the HLA-DQ2 and HLA-DQ8 haplotype or by a normal duodenal biopsy (Marsh 0) performed at endoscopy while on a gluten containing diet in individuals expressing the HLA-DQ2 (human leukocyte antigen) or HLA-DQ8 haplotype) * Abdominal or thoracic surgery. Exception: appendectomy * Gastrointestinal, endocrine or neurological diseases * Cardiovascular, respiratory, renal or urinary diseases * Hypertension * Diagnosed food or drug allergies * Psychiatric disorders * Eating disorders * Depressive disorders * Anxiety disorders * Psychotic disorders * Restraint or emotional eating * Dieters, especially no gluten-free diet or a diet low on gluten * Medication on a regular basis, exception: oral contraception * Smoking * History of cannabis use or any other drug of abuse for at least 12 months prior to the study * Alcohol abuse (more than 21 units of alcohol for men, more than 14 units for woman per week) * Pregnant or breastfeeding women